activity of human carbonic anhydrase

Dominant behaviours in the expression of human carbonic ...

2014-6-6 · Dominant behaviours in the expression of human carbonic anhydrase hCA I activity . M. Yahia M. Abdelrahim, a Muhammet Tanc, b Jean-Yves Winum, c Claudiu T. Supuran* c and Mihail Barboiu* a Author affiliations * Corresponding authors ...

1H-indazole molecules reduced the activity of human ...

Currently, carbonic anhydrase inhibitors are the target molecules in the treatment and diagnosis of many diseases. In present study, we investigated the inhibitory effects of some indazole molecules on the CA-I and CA-II isoenzymes isolated from human erythrocytes.

Esterase activity of carbonic anhydrases serves as ...

2018-6-9 · Carbonic anhydrase 9 (CA9) and carbonic anhydrase 12 (CA12) were proposed as potential targets for cancer therapy more than 20 years ago. However, to date, there are only very few antibodies that have been described to specifically target CA9 and CA12 and also block the enzymatic activity of their targets.

Carbonic anhydrase II increases the activity of the human ...

2007-5-4 · Carbonic anhydrase II increases the activity of the human electrogenic Na+/HCO3- cotransporter. Becker HM (1), Deitmer JW. Several acid/base-coupled membrane transporters, such as the electrogenic sodium-bicarbonate cotransporter (NBCe1), have been shown to bind to different carbonic anhydrase isoforms to create a "transport metabolon."

Carbon Dioxide Hydration Activity of Carbonic Anhydrase ...

Abstract. A stop-flow kinetic study was performed on the carbon dioxide hydration activity of the human carbonic anhydrase B isoenzyme carboxymethylated at its histidine 200, and of the human C isoenzyme carboxyketoethylated at its histidine 63.The Michaelis-Menten parameters determined between pH 5.6 and 8.7 showed striking differences between the native and the …

The Carbon Dioxide Hydration Activity of Carbonic …

The kinetics of catalysis of CO2 hydration by human carbonic anhydrases B and C (EC 4.2.1.1) has been reinvestigated with use of an improved pH indicator stop-flow approach that was checked by studying the uncatalyzed rate of hydration. The Michaelis-Menten parameters were determined between pH 5.8 and 8.8 in noninhibitory buffers. For both isoenzymes Km was …

The Enzymic Kinetics of Carbonic Anhydrase from Bovine …

2002-5-1 · Differences between high activity bovine carbonic anhydrase B and low activity human carbonic anhydrase B monitored through metal substitution. Journal of Molecular Catalysis 1984, 23 (2-3), 133-144. DOI: 10.1016/0304-5102(84)80001-2. Guozhi Zhou, Ming …

Carbonic Anhydrase Activity Assay kit (colorimetric)

2021-1-6 · loses its activity which results in a decrease of absorbance. This assay kit is simple and can be used to measure CA activity in biological ... Carbonic anhydrase-1, human recombinant (P1048) Carbonic anhydrase-8, human recombinant (P1047) E. coli Recombinant Carbonic anhydrase (P1049) ...

The Dinitrophenylation of Human Carbonic Anhydrase B

2020-11-5 · The COZ hydration activity of human carbonic anhydrase B is rapidly inactivated by 1-fluoro-2,4-dinitrobenzene at neutral PH. The inactivation reaction is second order and is retarded by the presence of various enzyme inhibitors. The second order rate constant for inactivation is about 100 ...

1H‐indazole molecules reduced the activity of human ...

2018-7-9 · Zuhal Alım, Zeynep Köksal, Muhammet Karaman, Evaluation of some thiophene-based sulfonamides as potent inhibitors of carbonic anhydrase I and II isoenzymes isolated from human erythrocytes by kinetic and molecular modelling studies, Pharmacological Reports, 10.1007/s43440-020-00149-4, (2020).

Catalytic activity of human carbonic anhydrase isoform IX ...

2015-10-16 · Introduction. Carbonic anhydrase isoform IX (CA IX), a transmembrane zinc metalloenzyme, catalysing the reversible conversion of CO 2 to bicarbonate and protons, has come to prominence in recent years because of …

Human Carbonic Anhydrase IX / CA9 (38-414) Protein, His ...

Carbonic anhydrase 9 (CA9 / CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell ...

Directed evolution of the promiscuous esterase activity of ...

2020-1-1 · Human carbonic anhydrase-II (hCA-II) is the most dominant physiologic isoform amongst the sixteen reported hCA isoforms. Because of its high availability in the different anatomical, and cellular sites of the eye like retina and lens, it plays a more prominent role in the regulation of intraocular pressure than the other twelve catalytically active hCA isoforms.

(PDF) The Carbon Dioxide Hydration Activity of Carbonic ...

The kinetics of catalysis of CO2 hydration by human carbonic anhydrases B and C (EC 4.2.1.1) has been reinvestigated with use of an improved pH …

Insight into the promiscuous activity of human carbonic ...

The promiscuous activity of human carbonic anhydrase (hCAII) against a non-physiological cyanic acid substrate has been investigated by using a combined QM and QM/MM level of theory. Results show that the hCAII is able to hydrate the cyanic acid by a reaction mechanism similar to that of the CO2 native subst

(PDF) The Dinitrophenylation of Human Carbonic …

The CO2 hydration activity of human carbonic anhydrase B is rapidly inactivated by 1-fluoro-2,4-dinitrobenzene at neutral pH. The inactivation reaction is second order and is retarded by the ...

Impact of Zn, Cu, and Fe on the Activity of Carbonic ...

2007-5-15 · The impact of zinc, copper, and iron on the duck erythrocyte carbonic anhydrase (CA) activity and the hemoglobin content in vitro culture were studied. The increase of zinc or iron addition at a low level induced the rise of CA activity, and the CA activity was inhibited by zinc or iron at a high addition level. The duck erythrocyte CA was strongly inhibited by cupric ion. The …

Directed Evolution of the Promiscuous Esterase Activity of ...

2019-5-25 · Human carbonic anhydrase II (hCAII) is an enzyme that evolved to catalyze the reversible hydration of CO 2 and performs this task at a remarkable rate (k ≈ 10 6 s-1). hCAII also exhibits promiscuous activity toward highly activated esters such as 4-nitrophenyl acetate. We describe a much weaker esterase activity of hCAII toward the ...

Function of Carbonic Anhydrase in Blood | Nature

CARBONIC anhydrase, which catalyses the reversible reaction CO2 + H2 ⇄ H2CO3, is an intracellular enzyme found in high concentration within red blood corpuscles1. The activity …

Prediction of activity and selectivity profiles of human ...

2021-3-6 · The development of selective inhibitors of the clinically relevant human Carbonic Anhydrase (hCA) isoforms IX and XII has become a major topic in drug research, due to their deregulation in several types of cancer. Indeed, the selective inhibition of these two isoforms, especially with respect to the homeostatic isoform II, holds great promise to develop …

Nitration and Iodination of Human Carbonic Anhydrases ...

O--O, nitration of carbonic anhydrase - -0, nitration of carbonic anhydrase C. Carbonic drase anhyR in enzymatic activity may be interpreted as arising either from denaturation of the protein or from iodination and inactivation of the active site of carbonic anhydrase B, where a histidine group is associated with enzymatic activity1 (16). 1 ...

The pH-Independence of Carbonic Anhydrase Activity ...

Carbonic Anhydrase Proton Acceptor Carbonic Anhydrase Activity Monovalent Anion Human Carbonic Anhydrases These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Redesign of human carbonic anhydrase II for increased ...

2010-3-15 · the esterase activity and specificity of human carbonic anhydrase II (HCAII) for esters with different acyl chain lengths was investigated. Following an initial screen of 7 HCAII variants with alanine substitutions in positions 121, 143 and 198, detailed kinetic measurements were performed on HCAII and the variants V121A, V143A and V121A/V143A.

Carbonic anhydrase activity in the frontal lobe of human ...

2021-3-8 · Carbonic anhydrase (CA) plays an important role in many biological processes. Recent technological advances have demonstrated the feasibility of measuring CA activity in the occipital lobe of human subjects in vivo.

Catalytic activity of human carbonic anhydrase isoform IX ...

2015-10-16 · Introduction. Carbonic anhydrase isoform IX (CA IX), a transmembrane zinc metalloenzyme, catalysing the reversible conversion of CO …

The Effect of Temperature on the Catalytic Activity of ...

Abstract. The two high activity forms of carbonic anhydrase which have been extensively studied, electrophoretic component C of the enzyme from human red cells and electrophoretic component B from bovine red cells, both show remarkably high catalytic activity.

Esterase activity of carbonic anhydrases serves as ...

Carbonic anhydrase 9 (CA9) and carbonic anhydrase 12 (CA12) were proposed as potential targets for cancer therapy more than 20 years ago. However, to date, there are only very few antibodies that have been described to specifically target CA9 and CA12 and also block the enzymatic activity of their targets.

Catalysis and Inhibition of Human Carbonic Anhydrase IV ...

1997-3-4 · Carbonic anhydrase IV (CA IV) is a membrane-bound form of carbonic anhydrase. We have characterized the catalytic activity and inhibition of recombinant human CA IV. CA IV is a high-activity isozyme in CO2 hydration with a pH-independent kcat value (1.1 × 106 s-1) comparable to that of CA II (8 × 105 s-1). Furthermore, CA IV is more active in HCO3- …